AN IMPROVED METHOD FOR THE ISOLATION OF ARTEMISININ FROM ANNUAL WORMWOOD (ARTEMISIA ANNUA L.) GROWING IN UZBEKISTAN

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  • English

    The chemical composition of representatives of the plant Artemisia annua L. in different geographical regions, in particular the amount of artemisinin, was studied. However, the chemical composition of its representatives growing in Uzbekistan has not been studied. The aim of this study was to isolate and quantify artemisinin from the plant Artemisia annua L. in Uzbekistan, especially in the Ferghana Valley.The extraction of artemisinin from the plant Artemisia annua L. in organic solvents, thin layer and column chromatography, IR, and mass spectrometry were used to determine the physicochemical parameters, chemical composition, and structure of the isolated substance.Isolation of artemisinin from the plant Artemisia annua L., growing in the Ferghana Valley, was achieved by 1.2-1.5% by replacing organic solvents. The extraction process with petroleum ether was carried out at different times. It was found that the yield did not change significantly when the extraction time exceeded 2 days. When analyzing the IR and mass spectra of the obtained substance, it was noticed that the data corresponds to the structure of artemisinin.For the first time, the biologically active substance artemisinin was isolated from the plant Artemisia annua L. growing in the Ferghana Valley in Uzbekistan. Extraction and purification methods have been improved, and the overall yield has been increased to 1.5%.

    Kalit so'z: #extraction #IR spectrum #COVID-19 #SARS-2-CoV-2 #coronaviruses #phytopreparations #Annual wormwood #Artemisia annua L. #artemizinin

  • Ўзбек

    Artemisia annua L. o‘simligining turli geografik mintaqalardagi vakillarining kimyoviy tarkibi, xususan, Artemizinin miqdori o‘rganilgan. Lekin, O‘zbekistonda o‘sadigan vakillarining kimyoviy tarkibi o‘rganilmagan. Mazkur tadqiqotda O‘zbekistonda, xususan, Farg‘ona vodiysida o‘sadigan Artemisia annua L.o‘simligidan Artemizininni ajratib olish va miqdorini aniqlash maqsad qilingan.Artemisia annua L.o‘simligidan Artemizininni ajratib olishda organik erituvchilarda ekstraksiya qilish, ajratib olingan moddaning fizik-kimyoviy ko‘rsatkichlari, kimyoviy tarkibi hamda tuzilishini aniqlashda yupqa qatlamli va ustunli xromatografiya, IQ, mass-spektrometriya usullari qo‘llanilgan. Farg‘ona vodiysida o‘sadigan Artemisia annua L.o‘simligidan organik erituvchilarni almashtirish orqali 1,2-1,5 % gacha Artemizininni ajratib olishga erishildi. Petroley efirda ektraksiya jarayoni turli vaqtlarda olib borildi. Ekstraksiya vaqti 2 sutkadan ortganda unum sezilarli o‘zgarmasligi aniqlandi. Olingan moddaning IQ- va mass-spektrlari tahlil qilinganda tuzlishi artemizininning tuzilishiga mos kelishi kuzatildi.Shunday qilib, birinchi marta O‘zbekistonda, Farg’ona vodiysida o‘sadigan Artemisia annua L.o‘simligidan Artemizinin biologik faol moddasini toza holda ajratib olindi. Ektraksiya va tozalash metodikasi takomillashtirildi hamda umumiy unumning 1,5 % gacha oshirilishiga erishildi.

    Kalit so'z: #extraction #IR spectrum #COVID-19 #SARS-2-CoV-2 #coronaviruses #phytopreparations #Annual wormwood #Artemisia annua L. #artemizinin

  • Русский

    Изучен химический состав представителей растения Artemisia annua L. в разных географических регионах, в частности количество артемизинина. Однако химический состав его представителей, растущих в Узбекистане, не изучен. Целью данного исследования было выделение и количественное определение артемизинина из растения Artemisia annua L. в Узбекистане, особенно в Ферганской долине. Для определения физико-химических показателей, химического состава и структуры выделенного вещества применяли экстракцию артемизинина из растения Artemisia annua L. в органических растворителях, методы тонкослойной и колоночной хроматографии, ИК, масс- спектрометрии.Выделение артемизинина из растения Artemisia annua L., растущего в Ферганской долине достигнуто 1,2-1,5% заменой органических растворителей. Процесс экстракции петролейным эфиром проводили в разное время.
    Было обнаружено, что выход существенно не изменился, когда время экстракции превышало 2 дня. При анализе ИК- и масс-спектров полученного вещества было замечено, что данные соответствует строению полыни.Впервые в Ферганской долине в Узбекистане из растения Artemisia annua L. выделено биологически активное вещество артемизинин. Методы экстракции и очистки были усовершенствованы, а общий выход увеличен до 1,5%.

    Kalit so'z: #extraction #IR spectrum #COVID-19 #SARS-2-CoV-2 #coronaviruses #phytopreparations #Annual wormwood #Artemisia annua L. #artemizinin

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